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(SPDP) CAS #: 68181-17-9 產品使用說明書（含氨基馬來酰亞胺蛋白）

SPDP，CAS #: 68181-17-9 產品可以偶連帶氨基的蛋白和巰基或馬來酰亞胺的蛋白，偶連帶活性基團的蛋白示意圖:

我們可以提供三種不同結構的SPDP異端功能蛋白交聯劑：SPDP,LC-SPDP,Sulfo-LC-SPDP

其中SPDP,LC-SPDP是脂溶性蛋白交聯劑，Sulfo-LC-SPDP是水溶性蛋白交聯劑。

Procedure for Conjugating Two Proteins, Oneof Which Contains Sulfhydryls

To conjugate between primary amines on oneprotein and sulfhydryls (-SH) already present on a second protein, only thefirst protein must be modified with the SPDP reagent.2 Then, after desaltingthe SPDP-modified protein to remove reaction byproducts and excess nonreactedreagent, the sulfhydryl-containing protein is added to make the final conjugate(Figure 2, Reaction 1)

Additional Materials Required

Water-miscible organic solvent such asdimethylsulfoxide (DMSO) or

dimethylformamide (DMF); not necessary forSulfo-LC-SPDP

Phosphate buffered saline with EDTA(PBS-EDTA): 100mM sodium phosphate, 150mM NaCl, 1mM EDTA, 0.02% sodium azide,pH 7.5

Proteins to be conjugated: In theprocedure, IgG and β-galactosidase represent the amine-containing andsulfhydrylcontaining protein, respectively.

Procedure

Equilibrate the vial of SPDP Reagent toroom temperature before opening. 2.

Immediately before use, prepare a 20mMsolution of SPDP Reagent:

Add 25μL of the 20mM SPDP solution to 2-5mgIgG dissolved in 1.0mL of PBS-EDTA.

Incubate for 30 minutes at roomtemperature.

Equilibrate a desalting column withPBS-EDTA, and buffer exchange the SPDP-modified IgG to remove reactionbyproducts and excess nonreacted SPDP reagent

Add 1-3mg of β-galactosidase (~1-3 molesβ-gal per mole of IgG) to the IgG solution and incubate the reaction mixtureovernight at room temperature

The proteins (IgG and β-galactosidase inthis example) are now conjugated.

Additional Information

Please visit the web site for additionalinformation on this product including the following items:

? Tech Tip #1: Attach a protein onto glass,silica or quartz surface using a cleavable crosslinker

? Tech Tip #26: Prepare a reversible andmeasureable sulfhydryl-reactive affinity column

Protocol For Pyridine-2-Thione Assay toDetermine Level of SPDP-modification

1. Dilute 100μL of SPDP-modified anddesalted protein to 1mL with PBS.

2. Measure and record the absorbance at343nm of the protein sample compared to PBS-EDTA blank (test in triplicate)

3. Add 10μL of 15mg/mL DTT to the 1mLSPDP-modified protein sample, mix.

4. After exactly 15 minutes, measure andrecord the absorbance at 343nm of the reduced sample.

5. Calculate the change in absorbance: ?A343= (Ave. A343 after DTT) – (Ave. A343 before DTT)

6. Calculate molar ratio of SPDP to Proteinusing the following equation:

Where the value 8080 reflects theextinction coefficient for pyridine-2-thione at 343 nm: 8.08 x 103 M-1 cm-1

產品名稱：SPDP

英文名稱：N-Succinimidyl3-(2-Pyridyldithio)propionate

中文名稱：3-(2-吡啶基二硫基)丙酸N-羥基琥珀酰亞胺酯

CAS#: 68181-17-9

簡介：SPDP crosslinker is a cleavable, waterinsoluble, amino and thiol (sulfhydryl) reactive heterobi-functional proteincrosslinker. The SPDP crosslinking reagent's reactive moites consist of anN-hydroxysuccinimide (NHS) ester that reacts with primary amines. On the otherend of SPDP, the pyridinyldisulfide reacts with sulhydryls to yield areversible disulfide bond.

SPDP交聯劑是可分解的，不溶于水，氨和硫醇（巰基）反應heterobi功能蛋白交聯劑。SPDP交聯試劑的反應的moites由N-羥基琥珀酰亞胺（NHS）酯與伯胺反應。在另一端的pyridinyldisulfideSPDP，反應sulhydryls產生可逆的二硫鍵。

Application

3-(2-Pyridyldithio)propionic acidN-hydroxysuccinimide ester (SPDP) is useful for the preparation of enzymeimmunoconjugates and hapten carrier molecule conjugates.

It has been used for antibody conjugation andas a protein cross-linker.

General description

3-(2-Pyridyldithio)propionic acidN-hydroxysuccinimide ester (SPDP) is a heterobifunctional cross-linking reagentwith amine and sulfhydryl reactivity. It is typically coupled initially tomolecules containing primary amines by amide bonds buffered at pH 7.5(6.5-8.5). Second coupling specific for molecules containing free sulfhydryl bythiol-disulfide exchange buffered at pH 8.0 (7.5-8.5). Alternately, this reagentmay be used as a protected thiolating reagent following initial coupling andreduction of linker. It incorporates four atom linker.

外觀：白色粉末

MolecularWeight: 312.4

分子式:C12H12N2O4S2

CAS#: 68181-17-9

溶解：DMSO or DMF

SpacerLength: 6.8 ?

Alternative Name:3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester

純度：98%+

保存：-20℃

廠家：西安瑞禧生物科技有限公司

產品名稱：Sulfo-LC-SPDP

英文名稱：(sulfosuccinimidyl6-[3′-(2-pyridyldithio)propionamido]hexanoate)

簡介：Sulfo-LC-SPDP crosslinking reagent isa cleavable, water soluble, amino and thiol (sulfhydryl) reactiveheterobifunctional protein crosslinker.

Sulfo-LC-SPDP crosslinker's reactive moitesconsist of an N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts withprimary amines. The other end of the Sulfo-LC-SPDP crosslinking moleculepyridinyldisulfide reacts with sulhydryls to yield a reversible disulfide bond.

LC SPDP交聯試劑磺可裂解、水溶性氨基和巰基反應異型雙功能交聯劑（巰基）蛋白。

SPDP交聯劑的反應moites磺LC組成的N-羥基（磺基NHS）酯與伯胺反應。的LCSPDP交聯分子pyridinyldisulfide磺化反應sulhydryls產生可逆的二硫鍵的另一端。

Caution

This product is sensitive to moisture. Thevial is packaged in a resealable bag with a desiccant to reduce exposure tomoisture. After cold storage, equilibrate the vial to room temperature beforeopening to reduce condensation inside the vial. Make fresh solutions. Storageof stock solutions is not recommended. After use, return the vial to theresealable bag. Close the bag and store the product at the recommendedtemperature.

General description

The SPDP reagents are a unique group ofamine- and sulfhydryl-reactive heterobifunctional crosslinkers. Whether theyare used to form amine-to-amine or amine-to-sulfhydryl crosslinks amongmolecules, the SPDP reagents produce disulfide-containing linkages that can becleaved later with reducing agents such as dithiothreitol (DTT).

外觀：白色粉末

MolecularWeight: 527.6

分子式：C14H16N3NaO8S3

溶解：水中

SpacerLength: 15.7 ?

Alternative Name:Sulfosuccinimidyl-6-(3’-[2-pyridyldithio]-propionamido) hexanoate

純度：98%+

保存：-20℃

廠家：西安瑞禧生物科技有限公司

產品列表：

Sulfo-NHS，N-羥基琥珀酰亞胺磺酸鈉鹽，CAS#: 106627-54-7

Biotin-ONP，(+)-生物素-4-硝基苯酯，CAS#: 33755-53-2

Biotin-PFP，五氟苯酚生物素酯/生物素五氟苯酯，CAS#:120550-35-8

6-(馬來酰亞胺基)己酸琥珀酰亞胺酯，CAS#:55750-63-5

6-Maleimidohexanoic acid，CAS#：55750-53-3

N-Maleoyl-β-alanine（MPA），CAS#：7423-55-4

4-Maleimidobutyric acid（MBA），CAS#：57078-98-5

11-馬來酰亞胺十一烷酸 N-琥珀酰亞胺酯，CAS#：87981-04-2

2-馬來酰亞胺基乙酸，CAS#：25021-08-3

11-Maleimidoundecanoic acid（KMUA）CAS#：57079-01-3

Sulfo-EMCS, ε-馬來酰亞胺己酸磺基琥珀酰亞胺酯  CAS#：215312-86-0

AMAS, CAS#：55750-61-3

1,2-Bis(maleimido)ethane, CAS#：5132-30-9

Boc-6-Ahx-OSu, CAS#：51513-80-5

N-Boc-2,2-(ethylenedioxy)diethylamine ,CAS#：153086-78-3

N,N,N,N-Tetramethyl-p-phenylenediamine,CAS#：100-22-1

N-Succinimidyl 6-Biotinamidohexanoate, CAS#：72040-63-2

N-(+)-Biotinyl-6-aminohexanoic acid  CAS#：72040-64-3

CAS#：127062-22-0

DTSSP

BS3  CAS#: 82436-77-9

BS2G

BSG             CAS#: 79642-50-5

DSP        CAS#: 57757-57-0

DSS        CAS#: 68528-80-3

DTSSP    CAS#: 81069-02-5

EGS       CAS#: 70539-42-3

Sulfo-EGS    

DSSeb    CAS# 23024-29-5

BMPS     CAS#: 55750-62-4

EMCS     CAS#: 55750-63-5

GMBS    CAS#: 80307-12-6

LC-SPDP      

MBS       CAS#: 58626-38-3

PDPH           

SBA       CAS#: 42014-51-7

SIA        CAS#: 39028-27-8

SMCC     CAS#: 64987-85-5

SMPB     CAS#: 79886-55-8

SMPH    

SPDP      CAS#: 68181-17-9

Sulfo-LC-SPDP    

Sulfo-MBS

Sulfo-SANPAH            CAS #: 102568-43-4

Sulfo-SMCC                 CAS#: 92921-24-9

Biotin Hydrazide           CAS #: 66640-86-6

NHS-Biotin                  CAS#: 35013-72-0

NHS-SS-Biotin             CAS #: 142439-92-7

Sulfo-NHS-Biotin

HPG                            CAS#: 24645-80-5

SATA                           CAS#: 76931-93-6

Sulfo-NHS Acetate        CAS #: 152305-87-8

Biotin-maleimide         

Biotin ethylenediamine hydrobromide  CAS# 216299-38-6

Biotinamidohexanoic acid hydrazide     CAS#: 109276-34-8

CAS#: 89889-52-1

TSAT

BMPH           CAS#:359436-61-6

EMCH           CAS#:151038-94-7

KMUH           CAS#:1593661-78-9

BMOE           CAS:5132-30-9

BMB              CAS#:28537-70-4

BMH (bismaleimidohexane)         CAS#:4856-87-5

TMEA

DTME           CAS#:71865-37-7

SATP             CAS#:84271-78-3

EMCA           CAS:55750-53-3

AEDP           

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